PHYTOCHEMICAL AND ANALYTICAL EVALUATION OF THE AERIAL PARTS OF THE THREATENED SPECIES CONDEA RIVULARIS.
Authors:
Lic. Madiley Pedraza Beltrán
Dr. Maria Elisa Jorge Rodríguez
Lic. Melissa Lliliam Gómez Saucedo
Summary
Introduction: Among the endemic and threatened species found in the central region of Cuba, Condea rivularis is one that has not had any study on its chemical composition and therefore its possible therapeutic use is unknown. Objective: To carry out qualitative and quantitative analyzes to determine the phytochemical composition of the aerial parts of the species Condea rivularis. Methodology: The pharmacognostic and phytochemical evaluation was carried out; in addition, the determination of metals, the determination of coumarin by thin layer chromatography and the development and validation of the high-performance liquid chromatography technique for the quantification of coumarins. Results: The numerical indices of the dry and milled drug support the quality of the plant material. The concentration values obtained for metals are within the accepted limits. The phytochemical screening showed the presence of fatty compounds, alkaloids, coumarins, phenols, among others. The development of thin layer chromatography evidenced the presence of coumarin. The high performance liquid chromatography technique turned out to be linear, precise, exact, specific and sensitive for the quantification of coumarins in the methanolic extract of Condea rivularis. Conclusions: Thin layer chromatography allowed to identify the presence of coumarin and the HPLC technique turned out to be linear, precise, exact, specific and sensitive.
Source: CITMA, CESAM, Villa Clara Environment Subdelegation
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